Characterization of Duplicate Genes in C. elegans
by Boeck, Max, Ph.D., UNIVERSITY OF WASHINGTON, 2011, 87 pages; 3501515

Abstract:

In this dissertation I focus on two different approaches to characterize functional differences between gene duplicates, focusing on those duplicates in C. elegans. First, I focused on two specific duplicates end-1 and end-3 and concentrate on deciphering specific defects in development caused by deletions in either gene. To do this, I take advantage of the invariant lineage of C. elegans. Using automated cell lineaging of fluorescently-labeled nuclei I find specific cellular defects in end-3(-) embryos as well as reporter gene defects for both end-1 and end-3(-). Second, I identify differences in gene expression patterns between duplicates on a genome-wide scale during development. I interpret these differences in gene expression patterns as evidence of diverged and unique functions. I conclude by discussing implications of these findings and further directions for research.

 
AdviserRobert Waterston
SchoolUNIVERSITY OF WASHINGTON
SourceDAI/B 73-07(E), p. , Mar 2012
Source TypeDissertation
SubjectsGenetics
Publication Number3501515
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