Calling, long present in theology, is a relatively new variable in organizational behavior studies. While researchers have yet to agree upon a common definition for calling, a factor analysis of the calling measure used in this study pointed to two components, transcendent purpose and prosocial orientation. Using a convenience sample of 204 individuals representing a wide variety of jobs, calling was hypothesized as a moderator of the relationships between job demands (measured as workload and job complexity) and burnout (measured as exhaustion and disengagement). The relationship between job demands and exhaustion was hypothesized as linear based on previous research, and the relationship between job demands and disengagement was hypothesized as curvilinear, based on activation theory (Gardner, 1986). In this model individuals experiencing low and high levels of job demands would experience higher levels of disengagement than individuals experiencing a medium level of job demands. No curvilinear relationship was found between job demands and the disengagement aspect of burnout, and no significant interactions between job demands and calling were found for either disengagement or exhaustion. Instead of acting as a buffer for job demands, both calling dimensions (transcendent purpose and prosocial orientation) showed main effects on both dimensions of burnout. The main effects of the two calling dimensions on exhaustion and disengagement have important implications both for the emerging understanding of calling theory and for the development of practical interventions to reduce burnout. The relationship between calling and disengagement is particularly relevant as widely effective interventions for this aspect of burnout have not yet been discovered.