The purpose of this research was to identify the impact system development methodologies may have on role ambiguity and role conflict experienced by information system developers. The primary research question addressed in this study was whether or not a correlation existed between role ambiguity, role conflict, and system development methodology use. Two mitigating factors considered during the course of this study were employee experience and the amount of time the system development methodology was employed by the organization. The primary research question and mitigating factors were divided into a total of five hypotheses, each of which was tested using the results of a survey sent to 1,000 software developers that belonged to the Institute of Electrical & Electronics Engineers (IEE). Based on the statistical analysis of the results of this survey, none of the hypotheses were tenable, which meant that the proposition that role ambiguity, role conflict, and system development methodology use were correlated was not supported by this study. One item of interest, however, was that the results of this study, compared to a similar study conducted over ten years ago, indicated that system development methodology use may have increased over the last decade rather than decrease as was predicted by the previous research.