This research study aimed to increase the body of knowledge and understanding of the relationship between employee perceptions of environmental and outcome factors and employee satisfaction for Baby Boomer and Generation X employees in the healthcare environment. The researcher performed a factor analysis of the raw data from an employee survey at a large nonprofit healthcare organization to determine whether selected variables that measure employee perception of work environment and outcome factors in the organization are influenced by generational cohort. Based on this analysis, the researcher determined the scales that best described the factors in the survey to develop a model for determining the work environment and outcome variables that determine employee satisfaction in a healthcare organization. The results of tests of regression indicated that there is no significant relationship between generational cohort and employee perception of satisfaction with job opportunities and a weakly significant relationship between generational cohort and satisfaction with work environment. Future study is recommended to provide an in-depth understanding of how the relationship between employee satisfaction, the organization, supervisors, and coworkers is able enhance the effectiveness of healthcare organizations in a highly competitive market.