This quantitative study made use of a descriptive cross-sectional non-experimental design to examine the relationship between the burnout rates of newly graduated registered nurses (RNs) and the number of preceptors they worked with during the clinical phase of their orientation to a first professional job. A secondary objective was to determine whether or not selected demographic and work-related variables would have a statistically significant correlation with burnout in new graduate RNs. To address these questions, the RNs were asked to complete the Copenhagen Burnout Inventory (CBI) and a demographic data form. Bivariate correlation procedures were conducted to ascertain whether there would be a statistically significant, positive relationship between number of preceptors worked with and burnout levels. Independent t-test procedures, one-way analysis of variance (ANOVA) tests, and bivariate correlation tests were conducted to assess whether there was a relationship between the demographic and work-related variables and burnout. The results showed a statistically significant relationship between number of preceptors and burnout; a statistically significant relationship between age and burnout; no statistically significant relationship between educational level and burnout; a statistically significant relationship between work shift and burnout; no statistically significant relationship between total number of weeks on orientation and burnout; and a statistically significant relationship between number of weeks working in the floor/unit of hire and burnout.