Abstract: Recent studies led to the proposal that meiotic gene conversion can result after transient engagement of the donor chromatid and subsequent DNA synthesis-dependent strand annealing (SDSA). Double Holliday junction (dHJ) intermediates were previously proposed to form both reciprocal crossover recombinants (COs) and non-crossover recombinants (NCOs) but are now thought to form COs, with SDSA forming most or all NCOs. In order to test this model, I constructed a random spore system in which it is possible to identify a subset of NCO recombinants that can readily be accounted for by SDSA but not by dHJ-mediated recombination. The diagnostic class of recombinants is one in which two markers on opposite sides of a double strand break (DSB) site are converted, without conversion of an intervening heterologous insertion located on the donor chromatid. This class represents 26% of selected NCOs. A second part of this project involved the use of another assay system in which two ends created by a single DSB must invade sequences separated by 40 kb in order to form a the selected recombination product. This system was constructed to examine mutants for defects in DNA end coordination, as axial structures anchoring chromatin loops are thought to constrain partner choice, thus coordinating the DNA ends and promoting allelic recombination at the expense of ectopic events; and also to lend evidence to the model that NCOs can arise from SDSA.