This study presents an examination of what has been commonly referred to as Cheikh Anta Diop's Two Cradle Theory. After a review of Cheikh Anta Diop's English-translated works which elucidate the Two Cradle Theory and a discussion of the Afrikan worldview methodological framework, this study then examines the written responses to the Two Cradle Theory among select Africana academics. Following this genealogy of the Two Cradle Theory within Africana academic discourse, the next task was to see in what ways discussion of Diop's Two Cradle Theory can contribute to the content, assumptions and organization of knowledge within Africana Studies. This study, therefore, argues that Cheikh Anta Diop's Two Cradle Theory is a multi-area theory within the academic discipline of Africana Studies. As a multi-area theory, the Two Cradle Theory can be used as the basis for the development of assumptions, concepts and interpretations within Africana psychology, Africana history and Africana philosophy. The concept of a multi-area theory was constructed as a discipline-specific and discipline-grounded concept within Africana Studies. Using Cheikh Anta Diop's Two Cradle Theory, as an example of a multi-area theory, this study shows its usability and consistency with previous scholarship in the discipline, along with future developments which are consistent with the organization of knowledge in Africana Studies.