Development of targeted phospholipid coated gamma-Fe2O3 nanoparticles as MR contrast agents

by Hultman, Kristi L., Ph.D., COLUMBIA UNIVERSITY, 2007, 119 pages; 3266601

Abstract:

Chapter 1: General introduction. A general overview of nanotechnology, nanoparticles and their applications is presented. The properties and characteristics of γ-Fe2O

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that make them excellent contrast agents are introduced, and an overview of magnetic resonance imaging and molecular imaging is given.

Chapter 2: γ-Fe2O3 iron oxide nanoparticles. The methods for synthesis, phospholipid coating, and conjugation to biological molecules are presented, along with techniques used for characterization of the resulting conjugate. Additionally, the specific binding ability of the antibodynanoparticle conjugates is demonstrated and optimized.

Chapter 3: Magnetic resonance relaxation properties and scan development. The contrast properties of iron oxide nanoparticles are examined. The relaxation rates, as a function of concentration, are determined. The scan sequences that were developed to optimize sensitivity towards the nanoparticles and produce clear images of the rat anatomy are presented.

Chapter 4: In vivo nanoparticle experiments. In vivo experiments are conducted on rats using a 3T MRI scanner to examine the lifetime and in vivo binding specificity of the targeted nanoparticles. The elimination time of the targeted nanoparticles in the kidney is measured, demonstrating the ability of the particles to behave as a targeted contrast agent.

Chapter 5: Conclusion. Future applications for the newly developed contrast agent are discussed.

AdvisersTruman R. Brown; Stephen O'Brien
SchoolCOLUMBIA UNIVERSITY
Source TypeDissertation
SubjectsBiomedical engineering
Publication Number3266601

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