Abstract: Actin based motility can produce protrusion and deformation of the cell membrane. Listeria monocytogenes, a food poisoning bacterium, moves with the cellular actin motility system producing propulsive force. The Listeria transmembrane protein, ActA, plays a crucial role in connecting the bacterial surface with growing actin filaments by interacting with the host cell's Arp2/3 complex and VASP creating the actin nucleation zone for propulsive force. We have constructed Listeria mimetic nanosized bio/inorganic hybrid particles employing nanofabrication and biotechnology. To stimulate directional control, we fabricated biomimetic particles asymmetrically with a ferromagnetic material to respond to an external signal to the engineered system. This nanosized artificial bacteria mediating actin motility system was encapsulated into microsized giant lipid vesicles forming artificial amoeba. Theoretically, we can explain the moving mechanism between the surface of biomimetic nanoparticle and treadmilling of actin filaments on the bacterial surface. Furthermore, the tape peeling model was used to demonstrate the crawling mechanism of the artificial amoeboid system. To elucidate the mathematic models of movement by biomimetic nanoparticles and the engineered amoeboid system, we performed motility tests with engineered vesicles containing biomimetic particles, actin, six kinds of actin binding proteins, ATP, and cations. This resulted in the mobile artificial amoeboid system crawling and showing typical actin based cell movement at a rate of 0.03 ∼ 0.22 μm/s. We could regulate the artificial amoeba movement by internal (biochemical) and external (magnetism) factors. For biochemical control, we regulated the actin monomer concentration and removed ATP in the motility media. The average speeds of the vesicles were changed with respect to the amount of applied actin monomers, and the ATP free vesicles did not show any motility. For controlled directional movement, we applied the magnetized field of 0.7 Tesla (T) to rearrange the biomimetic particles in the vesicles. Consequently, the ferromagnetic particle containing vesicles moved to the direction of applied magnetic field.