Abstract: This qualitative study explored the nature of psychospiritual transformation experienced by 12 mid-life women who walked over 2,000 miles on the Appalachian Trail (A.T.). Transformation was defined as a significant change in a person's functioning characterized as expanded self-understanding, a connection with Spirit, and a commitment to service. All but one participant walked the length of the trail during a 12-month period of time, and all identified themselves as deeply changed. The study incorporated two transpersonal research approaches: Organic Inquiry and Intuitive Inquiry. Evidence included the participants' embodied writing related to the wilderness of the A.T., spoken stories about their transformative experience, and created or chosen visual images of transformation. Their experiences matched Clements' (2000) description of transformation. Wilderness themes that emerged from the women's writing included encountering the embodied feminine, dissolving boundaries between the self and the wild, experiencing acceptance, being in relationship, everything as it should be, connected, challenging, trustworthy, sensual, vast, timeless, ever changing. Transformative themes that emerged and were portrayed through stories included a deep connection within the self, with others, and with the environment; feeling competent; trusting; a sense of presence; becoming an authentic self, desiring to be of service; experiencing a sense of wonder; and creativity. Stages in their transformation experience recalled the tale of descent and rebirth disclosed in the Sumerian myth of Innana. The women's experiences included beginning in darkness, arriving at the crossroads, the descent, experiencing compassion, retrieving the embodied self, experiencing rebirth, and coming home. The collective evidence strongly suggested the emergence of a more integrated and expansive level of psychospiritual development, accompanied by evidence of service and creative expression, following an extended encounter with wilderness. The study may have important implications for the field of women's studies, transpersonal psychology, and ecopsychology.