E. coli Engineered for Triglyceride Production
by Rucker, Joanna, M.S., TUFTS UNIVERSITY, 2012, 63 pages; 1512789

Abstract:

The goal of this work was to engineer Escherichia coli to synthesize and accumulate triglycerides (TG). Triglycerides, which are commonly sourced from plant oils, are substrates for products such as biodiesel, lotions, lubricants and cooking oils. A liquid chromatography-mass spectrometry (LCMS) method, developed to analyze the production of triglycerides by the engineered E. coli strains, demonstrated a yield of 1 mg/L triglycerides with 2 g/L dry cell weight (DCW) in LB medium containing 5 g/L glucose.

Triglycerides were produced from cell wall phospholipid precursors through engineered expression of two enzymes, PgpB and DGAT, which perform phosphatidic acid phosphatase activity and diacylglycerol acyl transferase activity, respectively. In addition, E. coli was engineered to express a third protein, phasin, which was hypothesized to improve triglyceride accumulation through stabilizing effects on hydrophobic lipid droplets in the cytoplasm.
 
AdvisersKyongbum Lee; Blaine Pfeifer
SchoolTUFTS UNIVERSITY
SourceMAI/ 50-06, p. , Jul 2012
Source TypeThesis
SubjectsCellular biology; Microbiology; Biochemistry
Publication Number1512789
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