The role of calmodulin-dependent protein kinase II in the regulation of biological rhythms in Paramecium tetraurelia
by McGee, Kayla J., M.S., INDIANA UNIVERSITY OF PENNSYLVANIA, 2011, 91 pages; 1502388

Abstract:

Paramecium tetraurelia are known to exhibit avoiding reactions (brief periods of backward swimming) in response to environmental stimuli that oscillate with a period of 61 minutes, indicating an underlying ultradian clock. The addition of various concentrations of KN-62, a drug known to inhibit CaMKII activity, disrupts the ultradian rhythm, decreasing the periodicity from 61 to 21 minutes and delays the increase in the frequency of avoiding reactions (the circadian rhythm) from 700 minutes in the control data to between 900-1050 minutes. RNA interference of the CaMKII gene also shortened the periodicity of the ultradian clock from 61 to 21 minutes and delayed the circadian rhythm from 710 minutes in the control to between 910-1050 minutes. These results indicate that CaMKII plays an important role in the regulation of these biological rhythms, and is the first study to show that CaMKII is involved in the regulation of ultradian rhythms.

 
AdviserRobert Hinrichsen
SchoolINDIANA UNIVERSITY OF PENNSYLVANIA
SourceMAI/ 50-03, p. , Dec 2011
Source TypeThesis
SubjectsMolecular biology
Publication Number1502388
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