The National Aeronautics and Space Administration (NASA) has been using the Transmission Control Protocol / Internet Protocol (TCP/1P) or a similar reference model for space communication. NASA considers switching over to the Delay Tolerant Networking (DTN) Protocol because of some limitations in space environments. As a DTN implementation, Interplanetary Overlay Network (ION) has been introduced by Jet Propulsion Lab (JPL) for space missions. According to the recent reviews, some work is carried out with the convergence layer protocol (CLP) of DTN as TCPCL or LTPCL in space communication. As of now, there has been no work done to investigate the performance of hybrid of ION protocols for a single file transmission, i.e. using one convergence layer protocol for one segment of network communication path and another convergence layer protocol for another segment of the path. In this thesis, we present an experimental investigation of hybrid of ION protocols with LTPCL, TCPCL, and UDPCL running over different segments of a typical three node, relay-types of interplanetary link to conduct file transfer experiments over a network test bed. The performance of different combinations of protocol stacks are compared to find a protocol stack, which gives the highest goodput over various interplanetary communication links. One major conclusion is that TCPCL|LTPCL has significant goodput advantage over all the protocols at long link delays especially at a high BER. The transmission with LTPCL protocol involved on long delays is more tolerable to long link delay and high BER.