Sp1 is a ubiquitiously expressed transcription factor found throughout the brain. Much research has been done characterizing the role of Sp1 in diseases like Huntington's and Schizophrenia, however little is known about Sp1's function in drug abuse and drug-induced neuroadaptations. Other transcription factors have been shown to play important roles in cocaine administration/abuse and also be implicated in dopamine neurotransmission. It is known that Sp1 binds with cofactors, either to facilitate baseline transcriptional activity or enhance transcription; however it is unclear if these cofactors modify transcription levels of downstream genes. Additionally, little is known about post-transcriptional modifications on Sp1, whether inhibitory or stimulatory, and their importance in Sp1's transcriptional regulation of downstream genes specifically after chronic psychostimulant exposure. This study seeks to examine whether Sp1 is dysregulated after prior chronic cocaine and withdrawal, and to identify key proteins involved in dopamine neurotransmission, differentially regulated on the transcriptional level by Sp1.