Integration mechanism of the site specific non-LTR retrotransposon, R2Bm
by Hull, Christi Lynn, M.S., THE UNIVERSITY OF TEXAS AT ARLINGTON, 2010, 52 pages; 1477606

Abstract:

The focus of this thesis is the mechanistic study of non-LTR retrotransposon replication using the site specific transposon R2Bm. Non-LTR retrotransposons integrate into new chromosomal sites by copying their RNA back into DNA at the site of insertion using an exposed chromosomal 3’ OH to prime cDNA synthesis.1 Although some of the early steps of an integration event have been detailed, how the element completes integration remains unknown.1 2 3 4 5 6 Clarifying the last integration step, known as second strand synthesis, is a major goal of this research. In order to accomplish this, R2 RNA and protein substrates were generated and used in biochemical assays to explore the aspects of RNP formation and second strand synthesis.

 
AdviserShawn Christensen
SchoolTHE UNIVERSITY OF TEXAS AT ARLINGTON
SourceMAI/ 48-06, p. , Jul 2010
Source TypeThesis
SubjectsGenetics
Publication Number1477606
Adobe PDF Access the complete dissertation:
 

» Find an electronic copy at your library.
  Use the link below to access a full citation record of this graduate work:
  http://gateway.proquest.com/openurl%3furl_ver=Z39.88-2004%26res_dat=xri:pqdiss%26rft_val_fmt=info:ofi/fmt:kev:mtx:dissertation%26rft_dat=xri:pqdiss:1477606
  If your library subscribes to the ProQuest Dissertations & Theses (PQDT) database, you may be entitled to a free electronic version of this graduate work. If not, you will have the option to purchase one, and access a 24 page preview for free (if available).

About ProQuest Dissertations & Theses
With over 2.3 million records, the ProQuest Dissertations & Theses (PQDT) database is the most comprehensive collection of dissertations and theses in the world. It is the database of record for graduate research.

The database includes citations of graduate works ranging from the first U.S. dissertation, accepted in 1861, to those accepted as recently as last semester. Of the 2.3 million graduate works included in the database, ProQuest offers more than 1.9 million in full text formats. Of those, over 860,000 are available in PDF format. More than 60,000 dissertations and theses are added to the database each year.

If you have questions, please feel free to visit the ProQuest Web site - http://www.proquest.com - or call ProQuest Hotline Customer Support at 1-800-521-3042.