This study was designed to evaluate and compare the anthropometric and fitness characteristics of levels of high school field hockey players based upon the position played so that high school coaches might adjust training to develop physical performance goals for positional characteristics, overall team improvement and potential collegiate participation. Thirty nine high school field hockey players participated in six fitness tests including One Repetition Maximum (1RM) Bench Press, 1RM Leg Press, Illinois Agility Run, Multistage Fitness Shuttle Run (MFSR), Vertical Jump, and 40 Meter Sprint in order to evaluate strength, agility, endurance, power, and speed. Players were categorized by field position. Height, weight and Body Mass Index were also recorded. All testing was completed during a two week period before the onset of preseason training. An ANOVA was used to determine significant differences between forwards, midfielders, defense and goalies followed by a Newman Keuls Post Hoc evaluation. Significant differences in player position were found in the endurance test MFSR as well as the lower body strength tests, 1 RM Leg Press test and 1 RM Relative Strength Leg Press, respectively. Although significance was not attained, there was a trend concerning the Illinois Agility run where forwards, midfielders, and defense appear to be more agile than goalies. There were no significant differences between positions in the 40 meter sprint, the One Repetition Maximum Bench Press (1 RM BP), or the Vertical Jump (VJ). However, mean scores indicate midfielders to have the highest sprint and VJ scores, while defense had the highest mean 1 RM BP. With high school female athletes being largely ignored in team sports research, the results of this study provide objective and quantifiable data on the positional differences in field hockey players. Further research may consider a larger population size to strengthen statistical results.