With Texas Christian University’s recent acquirement of a tropical research station in Costa Rica, plans for the station’s infrastructure as well as research structure are being made. Part of this plan is to make the research station sustainable. Because the research station relies on local streams as a water source, a sustainable water management plan is essential. While such a plan may take years to develop, a foundation for sustainable water use can be made by providing baseline data and suggesting an ongoing stream monitoring program. This project provides a baseline by measuring the (i) chemical, (ii) biological, and (iii) physical properties of two stream sites at the research station. The sites, assessed a priori as either pristine or impacted by grazing activity, were then analyzed based on the three parameters. The baseline data of this project also includes maps generated in geographic information systems, which are important in analyzing the regional basin. Based on the type of measurements taken in this project and the foreseen use of the research station, this paper suggests an appropriate stream monitoring program after consideration of three successful monitoring programs.