Experiments were conducted using bench-scale flow-through control devices that inactivate bioaerosols. These control devices used ultraviolet germicidal irradiation, which is UV-C radiation between 100–280 nm wavelength, to inactivate bioaerosols. Microorganisms exposed to this radiation experience damage to their DNA, rendering them unable to replicate. Three UV-C sources were tested: mercury, light emitting diode (LED), and xenon, as well as two wall reflectivities. Airflow rate through the device and relative humidity (RH) were also varied. In addition to measurements, modeling was performed to predict the effectiveness of the control devices. Measurements of inactivation rate constants, defined as a microorganism's susceptibility to UV-C inactivation normalized by the incident fluence rate, of Bacillus subtilis were made. At 50% RH, B. subtilis has an inactivation rate constant of 0.062 m²/J; at 15% RH the inactivation rate constant is 0.078 m²/J. The experiments determined that a high reflectance wall coating increases the fluence rate by a factor of 1.6. Modeling results showed good agreement when compared to experimental measurements with errors of less than 20%. Once the LED and xenon prototypes improve in functionality, they can be scaled up to full-scale applications like those currently using mercury lamps, such as heating, ventilating, and air conditioning (HVAC) systems.