Past research in ostracism has demonstrated that being excluded from a group has pervasive negative effects on an individual. This research addressed the degree to which group membership influences an individual’s response to being excluded either from an in-group or from an out-group. While previous research has suggested that the in-group/out-group distinction of the gender of source of the ostracism does not play a role in responses, the current research proposed that that exclusion from a source group that is central to social identity (gender) will pose a greater threat to the four needs than exclusion from a source group that is not. Previous research looking at the influence of the gender of source tend to focus on seemingly benign group memberships (Mac vs PC computer users in Williams et al., 2000, smokers vs non-smokers in Smith & Williams, 2004). Furthermore, group membership does not seem to have an effect on people’s responses even when the out-group is a hated out-group (such as the KKK in Gonsalkorale & Williams, 2007). The in-group/out-group domain focused upon in this study is the gender of the target of ostracism (participant) and the gender of the ostracism source (the people “interacting” with the participant). It was expected that this research would demonstrate that being ostracized would have negative effects on the four needs (self-esteem, sense of belonging, sense of control, meaningful existence) and mood. Consistent with previous research, being ostracized significantly lowered the participants’ four needs and mood. The expectation that the group membership of the gender of source of ostracism would impact an individual response was not supported. However, the participant’s level of gender identification did indicate a moderating affect of the relationship between the gender of source of the ostracism and being excluded.