Cystic Fibrosis (CF) is a genetic disease that affects multiple organ systems. The CF phenotype is associated with increased inflammation. Recent research has indicated a relationship between certain fatty acids and inflammation. Human bronchial epithelial cells (16 HBE) transfected with sense or antisense cystic fibrous transmembrane conductance regulator (CFTR) were grown in culture and supplemented with varying concentrations of n-3 and n-6 fatty acids. The cells were subsequently exposed to pseudomonas aeruginosa for one hour. Following removal of the p. aeruginosa, the cells were again supplemented with fatty acids for 20 hours. IL-8 secretion was measured via Enzyme-Linked Immunosorbent Assay (ELISA). Supplementation with linoleic acid increased IL-8 secretion in antisense cells. No change in IL-8 secretion was observed in cells supplemented with arachidonic acid. Supplementation with n-3 fatty acids, eicosapentaenoic acid and docosahexaenoic acid, resulted in a decrease in IL-8 secretion. However, this effect was not seen when results from multiple experiments were pooled. Identifying the relationship between n-3 and n-6 fatty acids and IL-8 secretion might benefit patients with classic cystic fibrosis, those with milder phenotypic diseases and those with chronic inflammatory diseases.