A novel regulatory pathway of transcriptional activation by mRNA cap-binding complex in vivo
by Bajwa, Pratibha, M.S., SOUTHERN ILLINOIS UNIVERSITY AT CARBONDALE, 2007, 64 pages; 1450003

Abstract:

In this thesis, we have explored two related aspects of eukaryotic gene regulation - (i) role of cap-binding complex (CBC) in transcription, and (ii) regulation of histone H3-K56 acetylation and its function in gene expression.

CBC binds to the mRNA cap structure to protect it from exonucleases. Intriguingly, CBC also regulates the downstream events such as RNA splicing, 3'-end formation and export. However, whether CBC plays a crucial role in controlling the upstream events such as transcriptional initiation or elongation, remains unknown. Here, we demonstrate that CBC stimulates the formation of the pre-initiation complex assembly at the active gene (and hence transcriptional initiation) in a positive feedback manner in Saccharomyces cerevisiae, thus providing a novel function of CBC in eukaryotic gene regulation.

In the second part, we have defined Rtt109p as a novel regulator of H3-K56 acetylation that is involved in maintaining genome stability, but not gene expression.

 
AdviserSukesh R. Bhaumik
SchoolSOUTHERN ILLINOIS UNIVERSITY AT CARBONDALE
SourceMAI/ 46-04, p. , Apr 2008
Source TypeThesis
SubjectsMolecular biology; Biochemistry
Publication Number1450003
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