Natural products extracted from plants have been the main focus of study in cancer research for the past 30 years. Colon adenocarcinoma is the third leading cause of cancer death in the United States killing an estimated 52,000 people in 2007. This figure emphasizes the importance of an effective treatment for colorectal cancer. The present study focuses on the preliminary survey of in vitro effects of extracts of Rumex crispus root on the human colon adenocarcinoma cell line, DLD-1. Rumex crispus root was collected locally, powdered and subjected to Accelerated Solvent Extraction, Solid Phase Extraction, and High Performance Liquid Chromatography in order to separate nonionic detergent (Triton-X 100) soluble compounds from the root. The SPE water flow-through and Triton-X eluate displayed a positive killing effect on the DLD-1 cells. The SPE samples were separated via HPLC and fractionated according to the compound solubility in acetonitrile on a reverse phase C-18 column. Many of the HPLC fractions showed positive killing of the DLD-1 cells to varying degrees giving monophasic to multiphasic dose response curves. Therefore it appears that Rumex crispus root contains several different active compounds capable of killing the DLD-1 cells. The results of this study provide evidence of toxic compounds available in the root of Rumex crispus with the potential to have anti-cancer activity.