Mobilization of nucleosomes in short arrays
by Sabri, Abdellah, M.S., SOUTHERN ILLINOIS UNIVERSITY AT CARBONDALE, 2006, 96 pages; 1440160

Abstract:

DNA is compacted into chromatin in eukaryotic cells. The histone-modifying enzymes and the enzymatic activity of the ATP-dependent chromatin-remodeling proteins are often involved in the disruption of the chromatin structure to allow access for the molecular machines that use it as a substrate for transcription, replication, recombination and repair.

DNA-protein site directed mapping showed that various chromatin remodeling complexes slide nucleosomes within di and tri-nucleosome arrays differently. ISW2 can translocate (slide) nucleosomes within an array up to 30 bp from the adjacent nucleosome. ISW2 slides nucleosomes towards the DNA ends in contrast to INO80 that slides the nucleosomes away from the DNA ends and introduces 30 bp spacing between nucleosomes. SWI/SNF slides nucleosomes apart within a di-nucleosome array. In addition to the sliding, SWI/SNF demonstrates capacity to evict an H2A-H2B dimer as an intermediate step, followed by eviction of one nucleosome.
 
AdviserBlaine Bartholomew
SchoolSOUTHERN ILLINOIS UNIVERSITY AT CARBONDALE
SourceMAI/ 45-02, p. , Mar 2007
Source TypeThesis
SubjectsMolecular biology
Publication Number1440160
Adobe PDF Access the complete dissertation:
 

» Find an electronic copy at your library.
  Use the link below to access a full citation record of this graduate work:
  http://gateway.proquest.com/openurl%3furl_ver=Z39.88-2004%26res_dat=xri:pqdiss%26rft_val_fmt=info:ofi/fmt:kev:mtx:dissertation%26rft_dat=xri:pqdiss:1440160
  If your library subscribes to the ProQuest Dissertations & Theses (PQDT) database, you may be entitled to a free electronic version of this graduate work. If not, you will have the option to purchase one, and access a 24 page preview for free (if available).

About ProQuest Dissertations & Theses
With over 2.3 million records, the ProQuest Dissertations & Theses (PQDT) database is the most comprehensive collection of dissertations and theses in the world. It is the database of record for graduate research.

The database includes citations of graduate works ranging from the first U.S. dissertation, accepted in 1861, to those accepted as recently as last semester. Of the 2.3 million graduate works included in the database, ProQuest offers more than 1.9 million in full text formats. Of those, over 860,000 are available in PDF format. More than 60,000 dissertations and theses are added to the database each year.

If you have questions, please feel free to visit the ProQuest Web site - http://www.proquest.com - or call ProQuest Hotline Customer Support at 1-800-521-3042.